Journal: Open Forum Infectious Diseases

Article Title: P-1810. Development of Avian and Human Influenza Analytical Reference Materials for Diagnostics and Surveillance

doi: 10.1093/ofid/ofaf695.1979

Figure Lengend Snippet: H5N1 qPCR data Figure 1: qPCR amplification curves generated with ATCC® VR-3436SD™ (subtype H5N1) (blue) and H5N1 gRNA (pink) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M.

Article Snippet: Figure 1: H5N1 qPCR data Figure 1: qPCR amplification curves generated with ATCC® VR-3436SDTM (subtype H5N1) (blue) and H5N1 gRNA (pink) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. Figure 2: H5N6 qPCR data Figure 2: qPCR amplification curves generated with ATCC® VR-3439SDTM (subtype H5N6) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. H5N1 qPCR data Figure 1: qPCR amplification curves generated with ATCC® VR-3436SDTM (subtype H5N1) (blue) and H5N1 gRNA (pink) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. H5N6 qPCR data Figure 2: qPCR amplification curves generated with ATCC® VR-3439SDTM (subtype H5N6) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M.

Techniques: Amplification, Generated, Multiplex Assay

Journal: Open Forum Infectious Diseases

Article Title: P-1810. Development of Avian and Human Influenza Analytical Reference Materials for Diagnostics and Surveillance

doi: 10.1093/ofid/ofaf695.1979

Figure Lengend Snippet: H5N6 qPCR data Figure 2: qPCR amplification curves generated with ATCC® VR-3439SD™ (subtype H5N6) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M.

Article Snippet: Figure 1: H5N1 qPCR data Figure 1: qPCR amplification curves generated with ATCC® VR-3436SDTM (subtype H5N1) (blue) and H5N1 gRNA (pink) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. Figure 2: H5N6 qPCR data Figure 2: qPCR amplification curves generated with ATCC® VR-3439SDTM (subtype H5N6) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. H5N1 qPCR data Figure 1: qPCR amplification curves generated with ATCC® VR-3436SDTM (subtype H5N1) (blue) and H5N1 gRNA (pink) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M. H5N6 qPCR data Figure 2: qPCR amplification curves generated with ATCC® VR-3439SDTM (subtype H5N6) using (A) a Hoffmann et al., 2016 assay targeting HA, and the (B) CDC Flu SC2 Multiplex assay targeting M.

Techniques: Amplification, Generated, Multiplex Assay

Journal: Veterinary Research

Article Title: Intestinal mucosal immune responses induced by oral administration of chitosan nanoparticles encapsulating the PEDV S1 protein

doi: 10.1186/s13567-025-01695-6

Figure Lengend Snippet: Preparation and characterization of S1-CS nanoparticles . A Effect of the pH of the chitosan solution on the solubility of the PEDV S1 protein. B Effects of the ratio of chitosan to S1 protein on the encapsulation efficiency (EE) and loading efficiency (LE) of S1-CS NPs. The dotted line represents the precipitation. C SDS‒PAGE analysis of the antigen content in S1-CS NPs. D TEM image of S1-CS NPs at 200 000 × magnification. E Cell viability evaluation of S1-CS NPs. As determined by the CCK-8 assay after 24 h. F Stability analysis of S1-CS in the simulated gastric fluid. CS, free S1 protein and S1-CS NPs were incubated with simulated gastric fluid (pH 1.2). All the data are presented as the mean ± SD.

Article Snippet: The African green monkey kidney epithelial (Vero)-CCL81 cell line from the ATCC and the PEDV QY-2016 strain (GenBank ID: MH244927) were preserved in our laboratory.

Techniques: Solubility, Encapsulation, CCK-8 Assay, Incubation